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TgMetreclin is a mouse liver-specific transgenic protein, which is expressed from the promoter of the mouse Metrnl gene. TgMetreclin is comprised of 1328 amino acids, including a mature protein of 1218 amino acids, and a putative signal sequence of 100 amino acids at the N-terminus (Krueger et al. 2009). Metreclin is a member of the ADAMTS protease family and contains the canonical catalytic residues, Asp48 and His154, as well as a unique Asn at the C-terminus, and is therefore a metalloprotease (Takahashi et al. 2009, 2011). Metreclin was shown to cleave human fibronectin and to have anti-angiogenic, anti-metastatic, anti-fibrotic and anti-inflammatory activity in various human and mouse models (Sakai et al., 2012, 2013, 2014, 2015).
TgMetreclin-C2366S is a point mutation of TgMetreclin, in which the cysteine residue at position 2366 is substituted with serine.
This mutation was based on a previous study showing that the cysteine residue at position 2366 of TgMetreclin is important for the catalysis of the proteolysis activity of TgMetreclin (Hiradate et al. 2012). This point mutation was expected to improve stability of the protein.
While the nucleic acid sequence of TgMetreclin-C2366S is the same as that of TgMetreclin, TgMetreclin-C2366S differs from TgMetreclin at amino acid residues 2366-2369, which are encoded by an ACG codon instead of ATG. In addition, TgMetreclin-C2366S contains an amino acid

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RuneScape was released on August 1, 2004 for the Java Platform. It has been a great success with over 10.9 million players at the end of the 2007 calendar year and 41.9 million by the end of 2008. It is likely the largest online game in the world, where it extends well beyond the PC.

On June 21, 2003, an alpha version of RuneScape was released to 36 registered users. Players were able to gain experience through a series of quests. On August 4, 2003, the game was updated to version 0.9.10, which made further changes to the gameplay and allowed players to trade items and skills with other players.

In June 2004, the first commercial version of the game was released. The game includes over 600 quests and is played in-game with the help of the RuneScape client.

RuneScape had the greatest commercial success of any MMOG. The game was first published by Jagex in 2004 and was available for download from Jagex’s website. In 2008 the game’s World of Warcraft-like MMOG was replaced by RuneScape 3, the game was then run by Sony Online Entertainment. An example of a Player-versus-Player (PvP) in-game.

Gamers are placed in a persistent world where they can interact with other players. Players can also learn the skills of other players by defeating them in one-on-one fighting or competitive PvP tournaments.

RuneScape is a free online game with a subscription fee of $10/month or $45/year. Before logging in, players must create their accounts. Once logged in, players can see their friends, guilds, servers, and availabilities.

In RuneScape, players must first create a character by choosing their name, gender, and appearance. The players cannot create their own character, but they can easily create a new account by applying the parent email address.

The game is very simple. The main goal is to explore the world, find resources, and level up. Players must also complete their quests. There are many classes of each character, in addition to other equipment.

Players begin the game on a randomly-selected server. Each server has its own geography and zone, which consists of many locations and NPCs. Players can explore the zone and keep logging in to update their progress.

Players can also fight for their lives in PvP battles or PvE dungeons
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