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Fungi are an important component of many wastewater treatment systems, where they play a key role in the degradation of biodegradable organic matter and toxic compounds. In this study, we evaluated fungal metabolism in continuous-flow bioreactors in a range of salinity (0.2-4 g l-1) and organic carbon concentration (1-10 g l-1) conditions. The following sources of organic carbon were used: an extract derived from beer brewing residue, an extract derived from a municipal wastewater plant, and laboratory media simulating municipal wastewater. The study involved the sampling of influent and effluent samples for chemical analyses, DNA extraction and polymerase chain reaction (PCR), and sequencing the PCR product. After DNA extraction and PCR amplification, sequence information could be linked to morphological and physiological grouping. With a PCR method that allowed the targeted analysis of a range of organisms in a single sample, the most abundant fungi were filamentous black yeasts in the effluent of all experiments. Diatom sequences were detected only in the influent of the second series of experiments. PCR-based sequencing of rDNA was a more sensitive and reliable technique for detecting changes in fungal abundance than using morphological and physiological grouping. Variation in fungal abundance is linked to the characteristics of the organic carbon source, the salinity of the wastewater treatment system, and the age of the wastewater sample (fresh or aged).Share this article on LinkedIn Email
Jolyon Palmer says he will not be blocking Fernando Alonso in the championship battle as he believes the Spaniard will not be fast enough to cause the damage to his Renault-powered McLaren.
Alonso came under attack in Spain following the British Grand Prix for lapping team-mate Kimi Raikkonen, and both drivers have been summoned to appear in front of the FIA’s World Motor Sport Council in Switzerland later this month.
Palmer is the only driver to have raced against Alonso previously, and the British team insists that should not deter it from working with him again.
“I am not going to block Fernando,”
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Capital Partners: College Prep and Beyond
K-12 schools would benefit from a transition to a college-ready,
four-year-old system, a new study by the Center for American Progress, the
Tocqueville Institution and the Center for College and Career Readiness
(C3R) concludes. In total, the authors estimate, a nationwide transition
to that system would save more than $8.5 billion by
On the one hand, the study shows that current efforts to prepare children
for the postsecondary years, like the No Child Left Behind Act, are
improving the academic skills of students. On the other, the study notes,
the transition could eliminate the need for four-year-old classrooms in
this country for years to come, if schools were to adopt effective teaching
and learning methods taught by effective educators.
The study’s main findings include:
2008, the typical high school senior in the United States could earn a
Bachelor’s degree in less than four years. This achievement, which
requires completing a combination of rigorous coursework and middle and
high school attendance, was consistent for students across all their